Abstract: Two edible mushrooms (Basidiomycetes, a macrofungus), Agaricus bisporus and Pleurotus sajor caju were assayed in vitro for their antimicrobial activities using aqueous and organic solvents extracts. The present study showed that Escherichia coli 390, Escherichia coli 739, Enterobacter aerogenes, Pseudomonas aeruginosa and Klebsiella pneumoniae were most sensitive to aqueous, ethanol, methanol and xylene extracts of these mushrooms. Thus indicated that the daily intake of mushroom can provide a natural covering of antibiotics to fight against the common pathogenic organisms.