Abstract: Biotechnology has offered important and efficient means for improving human life and health. However in spite of incredible development of biotechnological procedures, there are problems in point of economical view, especially in the case of products which are needed in huge amounts and relate to human health, such as vaccines. Application of biotechnology in such way that eliminates or reduces time-consuming and expensive processes, regarding production and subsequent quality control steps, can help better vaccination programs for large population, especially in the developing countries. The aim of this study was to summarize all data about human plant-based vaccine development including candidate antigens, transgenic plants and corresponding immunological responses in animal models or human using complete literature bibliography. The conclusion is that viral vaccines have been studied more than bacterial ones. Crude extracts of transformed plant materials as well as purified recombinant antigens expressed in plants have been found to induce immunological response in some investigations. Most of animal studies have been done with great success. Although few studies have been performed in humans but most of them have lead to hopeful results. Presently none of the commercially available products are produced in plants while most of biotechnology products which are comprised of proteins and possibly DNA-based vaccines are good potential candidates for plant-based production. Continuing investigations on plant-based vaccines is very crucial.