Abstract: In this study we aimed to examine the inhibitory activity of Vit C and mannitol on induced cytotoxic effect of “glycated protien-metal ion” system. Albumin was glycated by treatment with glucose for 2, 4 and 6 week incubation. After measurement of glycation, glycated albumin was added to rat-hepatocye suspension in presence and absence of either manntiol and Vit C. Produced MDA was measured in these systems as an indicator of oxidative stress and the results were analysed using descriptive statistics and Wilcocson matched pairs test. The obtained data showed that presence of Vit C in cell suspension leads to reduction of MDA (p = 0.000). Similar effect was observed for mannitol. Both substances showed stronger activity at 1.0 micromolare concentration. We concluded that using antioxidant such as Vit C and mannitol it is possible to diminish reactive oxygen species production in vitro. To extrapolate this effect in diabetic patients in vivo experiments are needed.