Abstract: Eurycoma longifolia (EL), a medicinal plant with pro-androgenic effects was recently reported to possess anti-osteoporotic activities. However, its effect on osteoporotic fracture has never been studied. This study aimed to provide more insight into the action of EL on healing of osteoporotic fracture by assessing callous strength. Tibial fracture was induced in orchidectomised rat, the model for osteoporotic fracture in androgen-deficiency state. The effects of EL supplementation for 6 weeks on fracture healing was assessed using Instron, a biomechanical testing device. The results were compared with testosterone, the standard treatment for androgen deficiency. Based on the biomechanical parameters, EL did not significantly improve the strength of fracture callous compared to testosterone replacement. These findings were not supportive of the role of EL in promoting osteoporotic fracture healing in orchidectomised rat model. To discover the true potential of EL, studies should be repeated with different castration models with intact testes for testosterone production.