Abstract: Spices and condiments consumed in Indian diets are one of the sources of phytochemicals and may act as antigenotoxicants. This study was taken up to evaluate the antigenotoxic potential of ginger in human peripheral blood lymphocytes. Peripheral blood samples from apparently normal 18 males (9 male smokers and 9 male non smokers) and 9 females were obtained. 0.5 mL of whole blood in a total volume of 5 mL medium was cultured in the presence of 0.16 mM Trans Stilbene Oxide (TSO) for the induction of micronuclei. Aqueous ginger extract was added at concentrations namely 0.1, 0.2 and 0.4 mg to test for antigenotoxicity. After 72 h, the cultures were processed and the cell suspension was fixed on slides and stained with 2% giemsa. The micronuclei were scored in 1000 binucleated cells and the data was analysed by analysis of variance (ANOVA). It was found that at basal condition without ginger and TSO the Micronuclei frequency was higher in smokers compared to non-smokers. In vitro treatment of peripheral blood lymphocytes with TSO induced significant number of micronuclei in all samples. Simultaneous treatment with ginger extract at all the concentrations significantly p<0.001 inhibited formation of micronuclei. A dose response relationship was observed. The extent to which ginger extract reduced the micronuclei formation induced by TSO exposure was almost normal or less than the control values. However, there were no changes in the vit. A and vit. E levels in all the groups. Results of the study indicate that ginger may protect against chromosomal damage induced by genotoxicant, as evidenced by this in vitro experimentation.