Abstract: This study aimed to investigate the cancerogenic effect of salted-fish samples (Chalcalburnus tarichi) on male and female mice. Eighty mice were fed with either salted fish and standart rat pellets separately (n=40) or only standart rat pellets (n=40) for 383 days. It was observed that the whole salted fish was eaten hungrily by mice. One mouse from each group and each sex were sacrificed in every month and liver, heart, esophagus, stomach, intestines, kidneys, testes and breasts were examined histopathologically. Serum BRMA (CA 15-3) (for breast cancer), GI-MA (CA 19-9) (for pancreatic and colo-rectal cancer), OMMA (CA 125) (for ovarian cancer) and CEA (for colo-rectal cancer) levels were measured as tumor markers. There was no histopathological or biochemical evidence of GI cancer in mice fed with salted fish. Only one animal developed lung cancer as a malignancy. Salted fish appears to have no association with GI cancer in mice.