Abstract: The isolate of Bacillus atrophaeus JS-2 (GeneBank accession number JN409601) was found to produce a bacteriocin which showed antimicrobial properties against Bacillus cereus, Bacillus licheniformis, Bacillus megaterium, Escherichia coli, Salmonella typhimurium and Aspergillus niger. There was prominent proteolytic activity with some lipolytic activity. The antimicrobial activities were seen between pH 5-8 but optimally at pH 5 and when the organism was grown at 40°C. During purification of the compound by precipitating with 80% ammonium sulfate, it was found to be associated with other proteins which were seen in the DEAE cellulose ion exchange chromatography. Characterization by SDS-PAGE showed single band at 43 kDa which was both proteolytic and lipolytic.