Abstract: Background: In Egypt, Florida prince peach an early harvest cultivar that adapt to local environmental conditions and potential for exportation. Color is variable as the global maturity indicator in peaches. Materials and Methods: In this respect, influence of ringing main branches technique at the beginning of coloring (4 weeks before harvest) with pre-harvest application of lysophosphatidylethanolamine (LPE) 2 weeks before harvest on quality and storability of Florida prince peach fruits were investigated during two successive seasons 2016 and 2017. The fruits were stored 30 days at 0°C±1 with 90-95% RH and fruit quality was evaluated at harvest and during cold storage. Results: At harvest, all treatments were more effective to increase fruit firmness, total soluble solids, total sugars content, color hue angle (h°) compared to the control in both seasons. While, produced a lower total acidity, total phenols, the activity of peroxidase and polyphenol oxidase. Results showed that by increasing the storage periods weight loss, decay, total soluble solids and total sugars content, the activity of peroxidase and polyphenol oxidase significantly increased. Whereas, fruit firmness, total acidity, color hue angle (h°) and total phenols significantly decreased. The best results obtained by sprayed LPE at 150 mg L–1 with ringing main branches after 30 days of cold storage which decreased fruits weight loss, decay, delayed the changes in total acidity, total phenols, activity of peroxidase and activity of polyphenol oxidase. Moreover, the same treatment produced a higher value of total soluble solids, total sugars and color hue angle (h°) compared with all treated fruits or the untreated ones. Conclusion: The study suggested that these treatments might be a promising candidate as to maintain peach quality and extending post-harvest life especially after cold storage.