Abstract: Glycine max L. (Soybean) is known for having its medicinal and nutritional value. It has capacity to accumulate high concentrations of cadmium (Cd). Studies were carried out to evaluate secondary metabolites production and biochemical potential of plant exposed to Cd-rich growing medium. Cd treatment in the form of Cadmium chloride (CdCl2) at supply of 0.25, 0.50, 1.00 and 2 mg L-1 increased the amino acid, protein, proline and diadzene content in field grown soybean plants at different developmental stages of Glycine max L. The enhancement was significant at Cd treatment of 1 mg L-1. The activity of antioxidant enzymes viz., superoxide dismutase (SOD), catalase (CAT) and Glutathione Reductase (GR) increased with increase in treatment of Cd in all developmental stages of soybean but increase in enzyme activity was more significant at Cd treatment of 2 mg L-1. Present finding suggested that higher diadzene production in cd enriched soyabeen contributed to higher medicinal value.