Abstract: An efficient plant regeneration protocol was developed for Santalum album L. (Santalaceae), an economically important species. Plant regeneration was achieved using internodal explants on Murashige and Skoog (MS) medium. Effect of Plant Growth Regulators (PGR) like 6-Benzyl Adenine (BA), Kinetin (KN) and 2-Isopentenyl adenine (2-iP) on shoot multiplication; 2-Isopentenyl adenine supplemented with varying concentrations of Coconut Milk (CoM) for shoot elongation and Indole-3-Butyric Acid (IBA) and α-Naphthalene Acetic Acid (NAA) on rooting was studied. Multiple shoots of S. album were significantly induced on internodal explants cultured on MS medium supplemented with 2-isopentenyl adenine (2iP) and Coconut Milk (CoM). The highest shoot multiplication was achieved on MS medium containing 1.0 mg L-1 (2iP) showed better growth response and produced 41.0±2.0 shootlets with an average length of 2.90±0.10 cm after 45 days of culture. The cluster of shoots were cultured on same media with 10% coconut Milk (CoM) showed shoot elongation up to 4.6 cm. Roots were induced after transfer to half strength MS medium supplemented with 0.5 mg L-1 IBA and 0.25 mg L-1 NAA produced 4.2±0.10 roots with an average height of 4.8±0.2 cm after six weeks in culture. The rooted plantlets were transferred for hardening, 70% of plantlets survived and resumed growth in the mixture of soil, vermiculite and farm yard manure (1:1:1).