Abstract: Twenty four peach genotypes from the Central North Region of Mexico, were characterized based on morpho-physiological traits. Fruit weights from the genotypes Roxana (135 g), San Gabriel C-167 (141.9 g) and Zacatecas landrace (162.3 g) were the higher, each in its group, since all genotypes were grouped according to their readiness to harvest as early-, middle- and late-harvest, respectively. RAPDs analysis yielded 52 monomorphic and 93 polymorphic fragments that were related to desirable characteristics from the Prunus genotypes. This information provide us tools for early individual identification of high-performance trees when still growing in the nursery. Therefore, growers may use this technique for assisted breeding program on their Prunus genotypes.