Abstract: Nodal explants of Salvia officinalis were grown on Murashige and Skoog (MS) media supplemented with various concentrations of naphthyl acetic acid (NAA), indole-3-butyric acid (IBA), benzylamino purine (BAP) and kinetine to induce adventitious shoots regeneration and micropropagation. The highest rate of growth was achieved on MS media supplemented with BAP (2.22 μM) (B05) and with kinetine (4.65 μM) and NAA (2.68 μM) (KN). The rooting process could be initiated only on KN medium. Histo-anatomical investigations revealed no significant structural modification on these media, especially on KN medium. At the same time, the non-rizogenetic action of NAA in Salvia officinalis was demonstrated.