Abstract: In this research, a karyological analysis on three naturally growing Limonium Miller species were made using Image Analysis System.The species studied are;Limonium iconicum (Boiss. and Heldr.) O. Kuntze,L. lilacinum (Boiss. and Bal.)Wagenitz and L. globuliferum (Boiss.and Heldr.) O. Kuntze. The chromosome numbers are determined as 2n = 34 in L. iconicum, as 2n = 36, in L.lilacinum and as 2n = 18 in L. globuliferum. The chromosome lengths in these taxa varied between 1.44 to 6.10 μm. The karyotype formula of the species studied were; in L. iconicum 10m+5sm+2T, in L. lilacinum7m+10sm+1T and in L. globuliferum 4m+5sm. The ideograms of each species were arranged according to the decrease in their length.