Abstract: The present investigation highlights the effect of plant growth regulators like gibberellic acid (GA) and Naphthalene acetic acid (NAA) on the yield and quality of bast fibres in Hibiscus sabdariffa. The fibre quality and yield was best in GA 100 + NAA 50 μg mL-1 treatment. Fibre macerate studies showed increase in fibre length and the slenderness ratio was also high. The Runkel`s ratio for the above treated fibre was between 1 and 2 rendering them suitable for textile industry. Proximate analysis of retted fibres revealed lower moisture and ash content and an increase in wax content. Fourier transform infra-red (FTIR) spectroscopy analysis registered a high crystallinity index. The physico-mechanical properties showed considerable improvement of fibre quality. Considering the above criteria, GA100+ NAA 50 μg mL-1 treatment brought about advantageous changes for improving the quality of fibres. This study is significant in promoting the exploitation of a non-conventional bast fibre source for use in textile industry.