Abstract: A micropropagation protocol was developed for a rapid production of Phyllanthus niruri (Euphorbiaceae) plantlets using nodal segments of the mature plants as explants. The aseptic nodal segments cultured on MS medium supplemented with 1.0 mg L-1 benzyladenine (BA) produced an average of 6.6 shoots from each explant. Continuous subculturing of the nodal segments enabled the production of healthy shoots. The addition of kinetin (KN) (0.5, 1.0 mg L-1) into the MS medium with the presence of BA (0.5, 1.0, 1.5, 2.0 and 2.5 mg L-1) induced multiple shoot formation in all (100%) the nodal segments. None of the nodal segments produced multiple shoots when they were cultured on MS medium supplemented with 0.5 or 1.0 mg L-1 KN without BA. Individual in vitro shoot of P. niruri could produce normal root systems when they were cultured on basic MS medium without any plant growth regulator. In vitro flowering and fruiting also occurred in 97% of the micro-shoots on MS medium without plant growth regulators. The first in vitro flowering was observed 12 days after initial proliferation of nodal segments while fruiting occurred 20 days after culture. The addition of gibberellic acid (0.5 mg L-1) into the MS medium was found to induce in vitro flowering within one week but inhibited in vitro fruiting. Basic MS medium supplemented with 30 g L-1 sucrose was found to promote healthy plantlet with 60 and 35% of the plantlets produced in vitro flowers and fruits, respectively within two weeks. All the in vitro plantlets produced flowers and 80% of them produced fruits after four weeks of culture on the same medium. The morphology of the in vitro flower and fruit were similar to that of the mother plant except they were smaller in size.