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International Journal of Botany

Year: 2006 | Volume: 2 | Issue: 2 | Page No.: 146-151
DOI: 10.3923/ijb.2006.146.151
In vitro Regeneration of Dryland Kenyan Maize Genotypes Through Somatic Embryogenesis
R. O. Oduor, E. N.M. Njagi, S. Ndung`u and J. S. Machuka

Abstract: In this study, we report successful regeneration of Kenyan white maize using immature embryos derived from three selected Kenyan dryland synthetic maize hybrids; Dryland Hybrid (DH) 02, Pwani Hybrid (PH) 01 and PH04 and an open pollinated variety-OPV (Katumani, KAT). The embryos were cultured in N6 medium supplemented with proline, casein hydrolysate, silver nitrate and 2% sucrose containing different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and incubated in total darkness at 28±2°C to initiate callus. Mature somatic embryos were sub-cultured in shoot promotion medium consisting of hormone free Murashige and Skoog (MS) media supplemented with 2 mg L-1 glycine and 2% sucrose. Root formation was induced by sub-culturing the shoots on half strength MS medium supplemented with 2% sucrose and 0.1 mg L-1 of Naphthalene Acetic Acid (NAA). Regenerated plantlets were successfully acclimatized in soil and raised to maturity. Fertile progeny were also recovered.

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How to cite this article
R. O. Oduor, E. N.M. Njagi, S. Ndung`u and J. S. Machuka, 2006. In vitro Regeneration of Dryland Kenyan Maize Genotypes Through Somatic Embryogenesis. International Journal of Botany, 2: 146-151.

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