Abstract: Background and Objective: Anthracnose caused by the pathogen Colletotrichum gloeosporioides penz is one of the major causes of post-harvest losses of mango in Côte d'Ivoire. Chemical control is a common method and has several side-effects. It is in this perspective that the present study was initiated with the general objective of evaluating the in vitro and in vivo antifungal efficacy of the biopesticides NECO 50 EC, ASTOUN 50 EC and PRORALY 50 EC based on plant extracts against the development of post-harvest anthracnose in mango. Materials and Methods: Five concentrations (100, 500, 1000, 1500 and 2000 ppm) of each bio-pesticide were evaluated in vitro on the mycelial growth of C. gloeosporioides. For the in vivo study, 250 mangoes of the Kent variety from the Poro Region, an anthracnose endemic area of Northern Côte d'Ivoire, were used. They were dipped in batches of 25 fruits (10 batches) in different bio-pesticides solutions at doses of 0.15 and 0.20% for 60 and 180 sec for the reference product (prochloraz) at the recommended dose of 0.025%. Treated fruits were stored in a cold room at 10°C for 15 days and at room temperature for 09 days. Results: The bio-pesticides NECO 50 EC and ASTOUN 50 EC completely inhibited the radial mycelial growth of C. gloeosporioides at concentrations of 1000, 1500 and 2000 ppm. Fruits treated with the biopesticide NECO 50 EC at concentrations of 0.15 and 0.20%, respectively showed no symptoms of anthracnose. In contrast, the negative control and the reference product had incidences above 10%. Conclusion: The present study showed the antifungal potential of the biopesticides NECO 50 EC and ASTOUN 50 EC in the control of post-harvest anthracnose of mango. They may constitute an alternative to the abusive use of synthetic fungicides.