Abstract: Background and Objective: Bio-fertilizer application has become one of the best alternates to chemical fertilizers, as an effective and sustainable soil management practice. Specific agronomy practices have been evolved in the cultivation of cotton, ultimately gained with improved yield and lint quality. An investigation was done to test the treatment effects over the growth, biochemical contents and cotton fibre yield and lint quality of a cotton cultivar, applied with Panchagavya and Trichoderma viride. Materials and Methods: Panchagavya and Trichoderma viride were applied to SVPR2 cotton cultivar, grown in containers under experimental conditions, using a randomized block design. Biochemical contents, yield response and lint quality were determined and the data were analyzed using biostatistical methods. Results: The biochemical contents analyzed in the cotton plants were found to have an influential role in the yield response and the physical quality of lint fibre, raised in the experimental SVPR2 cotton variety. Conclusion: The novelty in this research work, using the organic fertilizers treatment regime adapted has been found with an improved yield in cotton, combined with its better lint quality. This study result could be recommended to adopt organic farming for the benefit of cotton growers and industries and further contributing to the Cleaner Production Mechanism.