Abstract: Background and Objective: Barley is one of the ancient crop domesticated by humans and has been cultivated in marginal land due to its desirable characteristics like drought-tolerant With appropriate use of nitrogen fertilizer, the growth and development can be achieved at a higher level and thus to evaluate the effect of nitrogen fertilizer on yield parameters and yield on barley varieties, a field experiment was conducted to study the effect of nitrogen levels and varieties on yield attributes and yields of the barley crop. Materials and Methods: The experiment was laid out on a split-plot design with six levels of nitrogen (0, 20, 40, 60, 80 and 100 kg N ha–1) as the main plot treatment and four barley varieties (BH 946, BH 393, BH 885 and Karan 16) as a subplot treatment. Results: It was found that a higher level of nitrogen significantly influenced the yield attributes and was maximum when 100 kg N ha–1 were applied. The maximum grain yield (41.40 q ha–1) was recorded with the application of 100 kg N ha–1. The application of 20, 40, 60, 80 and 100 kg N ha–1 increases the grain yield by 44.83, 59.02, 67.13, 71.14 and 72.29%, respectively over control. Among varieties, the number of effective tillers and 1000 grain weight were found significantly higher in two-rowed barley variety BH 885. Whereas, BH 946 recorded the maximum number of grains per spike. The grain yield was significantly higher in BH 946 (33.11 q ha–1) which was at par with BH 393 but significantly higher than BH 885 and Karan 16. Conclusion: The study acknowledges the roles of nitrogen in imparting the yield attributes which are an important factor that decides the yield in a barley crop. It is also noted that varieties genetic makeup can influence yield and yield attributes given the same level of nitrogen.