Abstract: Background: Roselle have low genetic diversity, because its self-pollinating plant so it needs to be an effort to increase the genetic diversity of plants. The one of effort to increase genetic diversity is through mutation. Mutation induction of roselle plants aims to increase the genetic diversity of the plants that indicated by morphological changes and improve the character of the plant. Materials and Methods: Roselindo 2 variety seeds was used and evaluated the response on the growth and development of the first generation (M1) through gamma ray irradiation treatment. These seeds subjected to: 0, 100, 200, 300, 400, 500, 600, 700, 800, 900 and 1000 Gy to obtain the value of Lethal Dose 50 (LD50). Furthermore roselle seeds irradiated with doses of 0, 150, 300, 450 and 600 Gy to increase genetic diversity in roselle plants. Results: The results indicate that the value of Lethal Dose 50 (LD50) at 477.803 Gy. The variation derived from the number of branches and canopy diameter on M1 generation affects plant growth and development. Morphological changes occur in the form of branches, leaves and flower color than the control plants. Conclusion: Mutation induction with doses range of 0-600 Gy affects the growth and morphology diversity of roselle plants compared to plants without irradiation.