Abstract: Background and Objective: The Bacterial wilt caused by Ralstonia solanacearum (R. solanacearum) (Smith) Yabuuchi is a serious disease of tomato (Solanum lycopersicum L.) in tropical, subtropical and temperate areas of world including India and reduces the crop yields significantly. This study aimed to investigate the antibacterial activity through induction of defense-related enzymes by Bacillus amyloliquefaciens (B. amyloliquefaciens) DSBA-11 to manage bacterial wilt disease of tomato. Materials and Methods: Bacillus amyloliquefaciens DSBA-11was inoculated to two tomato cultivars Pusa Ruby (susceptible) and Arka Abha (moderate resistant) along with pathogen R. solanacearum UTT-25 at root zone of tomato under control conditions in Phytotronat 28°C and relative humidity was 67-75%. The one-way analysis of variance for biocontrol efficiency and yield of tomato was made by the SAS general linear model (GLM) procedure. Results: Minimum bacterial wilt intensity was recorded in Arka Abha (14.98%) treated with B. amyloliquefaciens DSBA-11 followed by Pusa Ruby (21.64%) which was significantly lower than the control treated only with R. solanacearum after 60 days of inoculation. Tomato seedlings of both cultivars cv. Pusa Ruby and Arka Abha treated with DSBA-11 produced significantly elevated production of superoxide dismutase (SOD), peroxidase (POD), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL) and catalase(CAT) as compared to control. Conclusion: The enhancement of induced systemic resistance enzyme activities in two tomato cultivars which could involve in suppression of the wilt disease.