Abstract: Oyster Mushroom [Pleurotus pulmonarius (Fries) Quelet] was cultivated on agricultural wastes viz., cotton, rice straw, corn cob, corn husk and sawdust. This study examined growth of Oyster Mushroom on these substrates with a view to increasing the substrate options in the production of mushrooms. Rice bran was added as a nutritional supplement to each substrate. Fruiting bodies were observed on different agricultural wastes after two weeks of incubation. Data collected were diameter of the cap [pileus (cm)], length of stem [stipe (cm)] and dry matter of fruiting body [sporophore (g)]. The dry matter (32.4±1.5) and pileus (19.2±2.4) of cotton waste supplemented with rice bran (CR) was significantly higher (p≤0.0001) while at the same level of significance, the stipe (18.0±1.2) for corn husk supplemented with rice bran (CHR) has significance difference compared with other substrates. The average width of the cap on the third day of appearance was highest for CR at 16% and lowest for Sawdust (S) at 6%; the average length of stem was highest for CHR at 15% and lowest for Corn Cob (CC) at 6% while dry weight was highest for CR at 27% and lowest for S at 2%. Supplemented substrates yielded better compared with non-supplemented substrates. CR was the best substrate followed by CHR while S was least. In addition to sawdust which is widely used by farmers, cotton waste, corn cob, husk and rice straw are possible agro-waste materials for oyster mushroom production.