Abstract: Forty nine anchote landrace populations collected from South and Western parts of Ethiopia were evaluated for 17 pheno-morphic and agronomic traits of yield and yield related traits in simple lattice design at Bako Agricultural Research Center during 2011 cropping season. The objectives of the study were to classify the population into relatively homogenous group and to identify the major traits contributing to the overall diversity of the population. The data were subjected to D2 analysis and the populations were clustered in to 5 different major groups according to their similarity levels and this makes the accessions to become moderately divergent. This dataset was reduced to three significant Principal Components (PCs) that cumulatively explained 93.50% of the variance. About 56.30% of the variance accounted for by the first PC alone resulted largely from the variations in contrasting effects of discriminatory traits like fruit yield per plant, fruit length, fruit weight, fruit diameter, hundred seed weight, petiole length, number of fruit per plant, leaf length, average root length, internodes length, vine length and root yield per plant. Overall, the study confirmed the presence of character diversity in anchote landraces. This assessment of traits diversity can assist geneticist and breeders to identify populations with desirable characteristics for inclusion in variety breeding program. Further evaluation at multi-location is suggested in the future.