Abstract: A study was undertaken to isolate biologically diverse strains of actinomycetes from medicinal plants rhizosphere soil collected from different locations of Yercaud hills belonging to Eastern Ghats of southern India for the production of bioactive secondary metabolites. The results indicated that a total of 20 strains were obtained from soil samples and further correlated with soil nutrients status in which five novel strains were screened for further investigation based on in vitro performance. The bioactive antimicrobial compounds were extracted from the purified cultures and tested their efficacy against some human pathogenic microorganisms such as Staphylococcus aureus, Escherichia coli, Bacillus amyloliquefaciens, Serratia marcescens and Pseudomonas fluorescence and beneficial microorganisms such as Phosphobacteria (PSB), Potassium solubilizing bacteria (KSB), Azospirillum and Rhizobium spp. including tea plant pathogens namely Phomopsis theae and Tunstallia aculeate. The results showed that all the strains were found to be of potential antagonists against the tested pathogens and thus providing the production of secondary metabolites.