Abstract: The present study was carried out to amplify glutenin genes to follow up any variations among different species of wheat genomes. Four designed specific primers of HMW-GS (HMW SX and HMW SY) and LMW-GS (LMW 7,8 and LMW S1) were used. In respect to the HMW-GS, the Subunit y gave higher polymorphism than subunit x, which failed to amplify gene patterns with diploid source of genome A and tetraploid species. While the polymorphism of subunit x appeared in variable size. In contrast, primers LMW 7, 8 and LMW S1 showed variations among the wheat species. The qualitative analysis of gene expression of HMW genes was studied by the SDS-PAGE, which was supported for the molecular studies and more informative approach to detect variations between and within species. Generally, the results of SDS-PAGE showed that, the absence or presence of GluA1 allele seemed to be responsible for clustering the cultivars and the species.