Abstract: The main objective of this study is to find out how xenobiotics (especially pesticides) bind to special protein and transported through the main living cell to reach its targeting. Because pesticides residues exists in foods, water, plants and underground then transported to human through the above sources, a lot of disease were detected in high intensity in the population in different countries. Therefore, research carried out to find out relationship between pesticides contamination in human breast milk and animal serum and protein profiles. Pollution with xenobiotics induce different form of cytochrome P-450 in human liver and directly accelerate the rate of α-synuclein fibril formation in living cell. Therefore, our investigation concern to find out the relationship between xenobiotics and protein binding and transportation in living cell. Breast milk and rat serum proteins as well as fish were used as examples of pollution materials with xenobiotics to find out the above relation.