Abstract: Field-based experiments were conducted to evaluate the combined application of Rhizobium sp. BHURC01 and plant growth promoting rhizobacteria on chickpea production in two consecutive years. A positive influence of plant growth promoting rhizobactria and Rhizobium sp. BHURC01 on nodulation, plant biomass, nitrogen and phosphorus in nodule, grain and straw and yield related parameter were recorded in two year of field experiments. The maximum significant increase in nodule number, dry weight of nodule, root and shoot were recorded in co-inoculation of Rhizobium sp. BHURC01 and Pseudomonas fluorescens followed by co-inoculation of Rhizobium sp. BHURC01, Azotobacter chroococcum and Bacillus megaterium over uninoculated control in two year of field study while, nitrogen and phosphorus content increase in nodules, grain and straw. The Rhizobium sp. BHURC01 and P. fluorescens showed significant increase in all parameter due to more available nitrogen by Rhizobium sp. BHURC01 and more available of phosphorus, iron and plant hormones like indole-3-acetic acid (IAA) and antifungal activity by P. fluorescens in comparison of B. megaterium and A. chroococcum. Therefore, co-inoculation of Rhizobium sp. BHURC01 and P. fluorescens could be effective biofertilizer for chickpea (Cicer arietinum L.) production.