Abstract: The endosperm storage protein allelic variations and agronomic performance in 85 accessions of persian wheat (Triticum carthlicum Nevski) were analyzed for their endosperm storage protein allelic composition. Higher variations of gliadins were observed in the persian wheat accessions and the gliadin variations among persian wheat accessions were associated with their geographic origins. At Glu-1 loci, 8 alleles were detected by SDS-PAGE analysis. At Glu-A1 locus, the subunit null was most frequently appeared (96.47%). At Glu-B1 locus, the most frequent subunits were 7+8 (95.29%). Moreover, the presence of subunits 14+15, 17+18 and 2*, which associated with good quality in bread wheat, were observed. The agronomic traits were evaluated to provide information for its utilization in breeding programs. All accessions were divided into five clusters based on principle component and cluster analysis. Every cluster had its particular characteristics. The cluster1 had higher spikelet numbers per spike and grains per spike and cluster 2 had higher 1000-grain weight with lower plant height. Most of the accessions in cluster 3 were derived from Turkey.