Abstract: The study was conducted to evaluate the effect of Asphedolus aestivus Brot. as a biopesticide against Tetranychus cinnabarinus Boisd. on toxicity, oviposition and hatching under the laboratory conditions. In the study, the root and leaf of A. aestivus were used and compared with dicofol. The root extract was found to be more toxic than the leaf one and the dosage was obtained at 250 gL/0.5 L. The results showed that the root extract had 46.6% mortality rate in 24 h and reached 53 and 57.1% at 2nd and 6th days. There were not seen any eggs in extract trials whereas it was 43 eggs in the control during 24 h. At 6th days the total amount of eggs was 20 in the extract and 63 eggs in the control. In other words, the extract reduced the eggs three times more. The eggs did not hatche through 6th days. However, it started hatching at 7th days and 4 times lower in the extract plot and total amount of eggs were the same at 9th days. It is concluded that A. aestivus has potential acaricide activity and inhibited oviposition and egg hatching. Finally, it can be used against T. cinnabarinus in Integrated Pest Management to reduce synthetic pesticide.