Abstract: The following physico-chemical parameters: hardness, moisture, Brix degree, ash, pH, acidity, ascorbic acid, total phenol compounds were determined in tomato samples belonging to Tyrlain cultivar cultivated conventionally, ecological and hydroponically (in tuff and cocoa fiber) and in several points of ripening. A clear tendency in the reduction of hardness and acidity and an increase of Brix degree/acidity ratio and ash was observed when the tomatoes ripened. The overripe tomatoes presented a lower ascorbic acid content than the green or commercial tomatoes. No detectable changes were found in the total phenolic compounds. Ecological tomatoes showed higher moisture content and lower Brix degree, ascorbic acid and total phenolic compounds than conventional and hydroponic cultivations. Hydroponic tomatoes presented a higher mineral content than those ecologically and conventionally cultivated. Many significant correlations were found between the physico-chemical parameters studied for the conventionally and the two hydroponically cultivated tomatoes. Hardness correlated positively with the acidity in all the methods of cultivation and after graphic representation, the overripe tomatoes tend to differentiate from the green and ripen tomatoes. Applying multivariate analysis the tomato samples tend clearly to differentiate according to the ripening stage and to a lesser extent, according to the type of cultivation.