Abstract: The aim of this research was to study the effects of the locally produced alternative substrates based on either river waste or Argentinean peat on germination and early growth of Verbena x hybrida in a plug production system. High and significant correlation coefficients between leaf area and initial pH, final electrical conductivity and final air-filled porosity were found. Root dry weight was adjusted better when related to initial electrical conductivity, initial porosity, final porosity and final air-filled porosity. The results indicate that river waste, Argentinean peat and rice hull are potentially substitute for Canadian Sphagnum peat in Verbena x hybrida plug production.