Abstract: The allelic compositions at Gli-A1 and Gli-A2 loci were investigated in 113 accessions of einkorn wheat by using Acid Polyacrylamide Gel Electrophoresis (APAGE). A total of 42 alleles were identified and the mean genetic variation index was 0.9418 at Gli-A1 and Gli-A2 loci in 113 accessions of einkorn wheat. A total of 37, 33 and 38 alleles were identified and the mean genetic variation indexes were 0.9378, 0.9314 and 0.9251 at Gli-A1 and Gli-A2 loci in the accessions of T. monococcum ssp. boeoticum, T. monococcum ssp. monococcum and T. urartu, respectively. In some accessions, the gliadin genes can`t be identified at Gli-A1 or Gli-A2 loci. It indicated that there might exist some new gliadin genes in these accessions. The results indicated that higher gliadin diversity was present in einkorn wheat. In addition, these accessions analyzed could also be used as genetic resource to improve the quality and to broaden the genetic base of common wheat.