Abstract: Background and Objectives: Recent statistics have shown a rise in the margin of consumption and utilization of essential oils. It has been recognized as some of the most promising compounds in the development of novel products. This study aimed to extract optimum amount of essential oil and investigation of antimicrobial activity of the oils from black cumin seeds. Materials and Methods: Extraction was carried out by use of steam distillation method in a Clevenger-type apparatus and the oils was established by using standard methods and the disc dilution method used for antimicrobial activity of oils and the test results were compared with standard antibiotics. Results: From the results obtained that, it was discovered that the cumin seeds could give the maximum yields of essential oil to be 40.4% w/w can be extracted by steam distillation at the optimum condition of temperature 96°C and time of 60 min. The seed essential oil was also tested for antimicrobial activity of the seeds oil against six pathogenic bacteria of E. coli, B. cereus, S. aureus, S. typhimurium, L. monocytogenes and E. faecalis show a maximal diameter of inhibition zone 19, 10, 12, 12,14 and 11 mm, respectively. The MIC values of these active plants seed extracts obtained in this study were lower than MBC values this suggests the seed extract were bacteriostatic at lower concentration and bactericidal at higher concentrations. Conclusion: Hence, the oil from cumin seed showed the highest antimicrobial activity against on all these pathogens bacteria and crude seed extracts are a mixture of different active compounds against bacterial strains. Therefore, the seed oil can be utilized for industrial and pharmacological purpose as well.