Abstract: Biodiesel was obtained from crude and refined rubber (Hevea brasiliensis) seed oil (RSO) by transesterification using different catalysts: sodium hydroxide (1), sodium metal (2), sulphuric acid (3), phosphoric acid (4), clay acid (5) and alkaline (6) activated. The yield, physico-chemical and fuel properties of the biodiesel were determined. The physico-chemical and fuel properties were compared to that of the commercial diesel fuel. The methyl ester yield from the crude RSO were in the order of III>IV>II>V>VI>I and that of the refined oil were of the order: II>I>III>IV>VI>V. On a general note, sample I formulated from the crude RSO which is sodium hydroxide catalyzed gave the least yield of 15% while sample II formulated from the refined RSO which is sodium metal catalyzed gave the highest yield of 92.1%. Comparative analyses of the properties of the biodiesel to that of commercial diesel fuel showed that transesterification improved the fuel properties of the oil. The viscosity, %free fatty acid values were reduced, while the calculated fuel potential increased. Other fuel properties were found to be in accordance with the ASTM standards.