Abstract: The chemical composition of the volatile oil from areal parts of Euphorbia thymifolia Linn. growing at Naini Patal, Distt-Pithoragarh, Uttarakh and was analyzed by GC-MS. The steam distillation of E. thymifolia areal parts ware carried out using a Clevenger apparatus in order to obtain the volatile oil (0.25%). It indicated presence of 30 compounds. The major constituents of oil were n-hexadecanoic acid (33.03%), phytol (10.36%) and tetradecanoic acid (6.58%). The oxygenated monoterpenes and sesquiterpene hydrocarbons were found in the oil as minor components. The yields of essential oil obtained from aerial parts were 0.05% (v/w). The nutrients, antioxidant and minerals composition in aerial parts of Euphorbia thymifolia Linn. were determined. Macro minerals viz., Na, K, Ca and Li were estimated by AIMIL, Flame Photometer while micro elements viz. Fe, Cu, Mn, Zn and Co were estimated by Atomic Absorption Spectrophotometer, model 4129, Electronic Corporation of India Ltd. The plants contained antioxidants such as β-carotene, Vitamin C and phenolics (307.40, 88.48 and 336.73 m/100 g), respectively. The plants have been reported rich in nutrients such as crude protein, carbohydrate, crude lipids, starch and crude fiber (13.42, 11.99, 4.63, 22.31 and 24.34 g/100 g), respectively. The plants have also been reported rich in minerals such as Na, K, Ca, S, P, Fe, Mn, Cu and Zn (75.40, 4786.48, 242.46, 325.24, 226.81, 121.04, 9.28, 3.45 and 6.47 mg/100 g), respectively. The study established the chemical composition of the essential oil of the plants. The plants have been found as a good source of nutrients, antioxidants and minerals. Results suggest aerial parts of plants could be used as raw materials in drug formulation.