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Current Research in Bacteriology

Year: 2023 | Volume: 16 | Issue: 1 | Page No.: 27-36
DOI: 10.3923/crb.2023.27.36
Characterization and Molecular Identification of Bacterial Isolates from Tail and Fin Rot Infected Silver Carp
Dilruba Afrin , Md. Ashikul Islam, Md. Nazmul Islam Bappy, Md. Shahadat Hossain , Kazi Md. Ali Zinnah, Biswanath Sikdar, Md. Asadul Islam, Md. Khalekuzzaman and Shamsunnahar Mukta

Abstract:

Background and Objective: Silver carp is considered one of the most cultivated farm fish of Bangladesh but bacterial diseases like fin and tail rot disease make it difficult for the farmers to maintain the silver carp properly. Therefore, this study tried to isolate and detect bacteria from the tail and fin rot disease-infected silver carp collected from the Sylhet Region of Bangladesh. Materials and Methods: Isolated bacteria were subjected to different morphological and biochemical tests for their preliminary identification. The recovered isolates were then sequenced for the 16S rRNA to confirm identification and to understand subsequent evolution using phylogenetic relationships. In addition, antibiotic sensitivity of the isolated strains was carried out to screen potential antibiotics against those foes. Results: The biochemical test and the molecular identification confirmed the presence of Kurthia gibsonii (Accession Number: MN658386), Klebsiella pneumoniae (Accession Number: MN658387) and Bacillus subtilis (Accession Number: MN658386) in the diseased fish. The antibiogram test revealed that all three isolates were susceptible to cefotaxime and kanamycin. However, the isolated strains also exhibited resistance to particular antibiotics at certain degrees which indicated the probable failure of those antibiotics to combat those pathogens. Conclusion: The detection and antibiogram profiling of isolated bacteria from tail and fin rot diseased silver carp will help to identify proper treatment options against this disease.

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How to cite this article
Dilruba Afrin, Md. Ashikul Islam, Md. Nazmul Islam Bappy, Md. Shahadat Hossain, Kazi Md. Ali Zinnah, Biswanath Sikdar, Md. Asadul Islam, Md. Khalekuzzaman and Shamsunnahar Mukta, 2023. Characterization and Molecular Identification of Bacterial Isolates from Tail and Fin Rot Infected Silver Carp. Current Research in Bacteriology, 16: 27-36.

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