Abstract: The effect of smoke produced by burning of neem leaves (Azadirachta indica) was studied for controlling airborne bacteria in four rooms of house premise viz., kitchen, dinning hall and two meeting halls in Dubai. Petri plates containing with bacterial agar medium were sterilized in an autoclave and placed at Cassella airborne bacterial silt for monitoring. Bacterial colonies developed in those petri plates which were exposed to ambient environment, whereas no bacteria colony were seen in petri plates exposed to neem leaves smoke treated environments. Maximum bacterial colonies were developed in kitchen followed by dinning hall and the meeting rooms (majlis) in the ambient environment. Neem leaves smoke treatment resulted in controlling bacterial growth on bacterial agar medium petri plates. Studied revealed that neem leaves smoke has antibactericidal properties and could be used for controlling airborne bacterial contamination in the residential premise.