Abstract: The main object of this study was to screen thermophilic bacterial strains with selective antimicrobial potential from garbage dump site. Six thermophilic bacterial strains were isolated from municipal solid waste by enrichment technique. All these strains were screened for their antimicrobial activity by using agar well diffusion assay against Gram-negative and Gram-positive bacteria. The methanol extracts of Cell Free Supernatant (CFS) of four bacterial strains Brevibacillus borstelensis, Bacillus galactosidilyticus and Bacillus licheniformis RH101 and RH104 were found to inhibit the growth of test organisms. The strain Brevibacillus borstelensis showed maximum inhibition against Micrococcus flavus, Dietzia sp. K44 and Staphylococcus aureus while Bacillus licheniformis strains and Bacillus galactosidilyticus inhibited the growth of at least one gram-positive test organism. The methanol extract of Brevibacillus borstelensis showed very selective antimicrobial activity against Gram-positive pathogens.