Abstract: Enzyme metabolism is a fundamental biological process that is vital for the survival of all species. Their specific function is to catalyze chemical reactions. Enzymes have found wide and diverse applications at which enzymes increase the rate of reactions which approach to equilibrium. Enzymes play critical role in the metabolic activities of all living organisms whether humans, animals, plants or microorganisms and are widely applied in microbialtechnology and their diagnosis processes. Abnormality of the enzyme metabolism system leads to a number of metabolic diseases. It is shown that many diseases associate with many components of the enzyme metabolism systems are now widely applied in clinical examinations as special markers for diseases. An interesting discovery suggesting that new roles of enzymes as a potential link that associates to prevent metabolic disorder. The aim of this review is to discuss the diverse diagnostic application of biotechnological enzymes for the purpose of teaching, education and research.