Abstract: The aim of this study was to determine the sequence of the 16S rRNA gene and thus to conduct the phylogenic position of the naturally occurring wild type strain of Streptomyces QU66C. Here we show conclusively the full sequence of the 16S rRNA gene of a novel wide type strain of Streptomyces coelicolor which has been isolated from the soil of Qatar and thus characterized on the basis of its phenotypic and genotypic features. In comparison with the homologous strains in GeneBanks, the phylogenic position of the isolate is in between S. coelicolor A3 (2) (Y00411) (NC003888) and S. coelicolor (C) (EF371438). The sequence of present strain has been deposited in the International Nucleotide Sequence Databases) (INSD) in the GenBanks/DDBJ/EMBL/NCBI) and assigned an accession number of AB588124 and thus the strain is being known as Streptomyces coelicolor (AB588124) (QU66C-2002). Present strain shows a similarity and identities of 99.40, 99.40, 99.40, 99.33 and 99.31% with a score value of 2693, 2693, 2687, 2673 and 2673 bits for Streptomyces coelicolor A3(2) (Y00411) (NC003888), S. violaceoruber AF503494 (ancient name for S. coelicolor and S. lividans), S. lividans AF503498, S. lividans AB184826 and S. coelicolor (C) (EF371438), respectively. In comparison with the S. coelicolor clones in Sanger database the strain shows a positive high scoring alignment similarity of 99.4% with score of 6907 with five clones of S. coelicolor A3 (2) (AL939116, AL939119, AL939124, AL939114, AL939108) and a 99% similarity with a score of 6889 for AL939110. Similarly, the BLAST search on EMBL GeneBAnk shows a 99.40% similarity with 2 strains of S. violaceoruber (AF503494, AF503492) with high score of 2738.