Abstract: Chitin, a linear N-acetylglucosamine is a structural component of fungal cell wall and exoskeletons of invertibrates. Chitinases catalyses the conversion of chitin and it is produced by a wide range of bacteria. The gene for chitinase biosynthesis Chi was PCR amplified from genomic DNA of Bacillus halodurans. The amplified genes were cloned and nucleotide sequences were determined. The sequencing results showed that Chi gene contain 1800 bp long ORF encoding 599 amino acids respectively (GenBank accession No. GU481106). Computational sequence analysis of nucleotides and amino acids revealed that the Chi sequences of Bacillus halodurans were conserved in many eubacteria.