Abstract: The objective of this study was to determine the quantitative expression of COR gene (Wcor14) in different cold treatment in Iranian winter-type wheat, using semiquantitative RT-PCR analysis. Semiquantitative RT-PCR analysis showed that Wcor14 specifically induced by low temperature. The transcripts of Wcor14 were up-regulated within 3-6 h of cold acclimation at 4°C.