Abstract: In this study, different tomato genotypes were transformed with silencing trigger construct that showed high efficiency in controlling Tomato yellow leaf curl virus. Cotyledons and hypocotyls of the tomato hybrid (981 X TY-6) were cultured on eight regeneration media containing different concentrations of auxins and cytokinins. Zeatin and Indole Acetic Acid (IAA) at 1 and 0.1 mg L-1, respectively, were significantly enhanced shoot regeneration. Different concentrations of Phosphinothericin (PPT) were used to select transformed tomato plantlets; the lowest PPT concentration (5 mg L-1) inhibited the growth of non-transformed plantlets but did not affect the growth of transformed plantlets. The inhibitory effects of four antibiotics (carbincillin, clavamox, cefotaxin and kanamycin) on the growth of Agrobacterium tumefaciens and the regeneration of shoots were investigated; carbincillin and clavamox at 500 mg L-1 showed high inhibitory effect. The transformation data indicated that the rate of regeneration from transformed tomato line (MP-1) was significantly higher than that of tomato hybrid (981 X TY-6).