Abstract: This research aims to select and evaluate the performance of three high-ethanol-producing strains of Saccharomyces cerevisiae (TISTR 5048, TISTR 5339 and NP 01) in very high gravity (VHG) ethanol fermentation. The maximum specific growth rates (μmax) of TISTR 5048 and NP 01 grown in yeast extract malt extract broth containing 150 g glucose L-1 were 0.49 and 0.46 h, respectively while μmax of TISTR 5339 could not be determined due to cell flocculation. The ethanol production by TISTR 5048 and NP 01 was further carried out in batch mode at 30 °C under normal gravity fermentation (240 g glucose L-1) and VHG fermentation (280 and 320 g glucose L-1) and the initial cell concentration was 1x108 cells mL-1. The results showed that TISTR 5048 cultured in 240 and 280 g glucose L-1 gave the maximum ethanol concentration (P) with the value of 99.58 g L-1, while NP 01 cultured in 280 and 320 g glucose L-1 gave the maximum P with the value of 104.68 g L-1. Ethanol productivities (Qp) of NP 01 were slightly higher than those of TISTR 5048 at all conditions tested.