Abstract: To produce transgenic plants which have various applications in agricultural and non-agricultural fields, a marker gene is necessary to recover a viable transgenic plant. To express or transcribe of transgenes, utilization of promoters is also unavoidable. Analysis of transgenes includes copy number, insertion site, integration stability, expression and it`s pattern and variability is immensely important in order to develop a successful transgenic event. This review presents the necessities for better recovery of transgenic plants, transcription or expression of transgenes, as well as methods to analyze transgenes.