Abstract: Genetic diversity in 19 local dry bean populations (Phaseolus vulgaris L.) originated from the region of Macedonia (Greece, GR and Formal Yugoslavic Republic of Macedonia, FYROM) was investigated using RAPD (Random Amplified Polymorphic DNA) markers in 10 individual plants from each population. A total of 11 random primers detected 56 polymorphic bands, with an average of 5.1 polymorphic bands/primer. The individual plants were grouped in 14 clusters on the basis of the Jaccard coefficient (Unweighted Pair Group Method and Arithmetic Average-UPGMA). The average taxonomic distances between the 19 populations were calculated on the basis of gene frequencies. These distances were used for grouping the populations, by UPGMA and Principal Coordinates Analysis (PCO), resulting in four and 10 groups, respectively. In general, there was a noticeable similarity in the grouping of the individuals and populations with the two methods. According the UPGMA and PCO procedures, the populations were clustered based on geographical origin. It was concluded that RAPD markers could be exploited as alternative or supplementary tools to already established methods for the evaluation and classification of bean genetic resources.