Abstract: This study aims to develop a molecular method easily applicable in Cote d=Ivoire for the International Coconut Genebank for Africa and Indian Ocean (ICG-AIO) finest characterization. A total of 62 individuals from eleven coconut accessions were used for microsatellite gene diversity analysis experienced. The mean number of alleles and the mean gene diversity within accession were 3.08"1.11 and 0.536"0.218, respectively for all accessions studied. By cluster analysis, accessions were structured into two main gene pools. Coconut accessions originated to south pacific were clustered together with the Sri-Lanka Green Dwarf accession and those from Africa grouped in a second cluster. Within the two groups, it was observed divergences between some accessions. These first results evidently indicated that microsatellite markers can be successfully applied in Cote d=Ivoire to study coconut resources of the ICG-AIO, as it=s important for ongoing efforts to facilitate local coconut resources characterization and conservation.