Abstract: The present study aims at isolation and characterization of biologically diverse strains of Streptomyces from soil samples for the production of bioactive secondary metabolites. There were 10 isolates obtained from different areas in Tamil Nadu and studied for the detection of antimicrobial substances contained it. These antimicrobial compounds were extracted and tested against some pathogenic microorganisms such as Staphylococcus aureus, Escherichia coli, Bacillus amyloliquefaciens, Serratia marcescens and Pseudomonas fluorescence as well as beneficial microorganisms like Phosphobacteria. The results showed that out of 10 isolates of Streptomyces sp. three of the isolates were found to be of potential antagonists against pathogenic organisms and thus providing the production of secondary metabolites which has the potential to control the soil borne pathogens. In contrast, some of the isolates showed no zone of inhibition with Phosphobacteria. This shows that it was not affected their growth.