Abstract: The reliability of Random Amplified Polymorphic DNA (RAPD) techniques to detect genetic diversity between Trochetia boutoniana species from six different locations in Mauritius was investigated. DNA fragments generated by 10-base primers were compared and variation could be detected. OPC-18 for example generated banding patterns unique to Trochetia boutoniana from three of the six locations. Investigation indicates that RAPD markers can be used to characterize Trochetia boutoniana species and that the technique is sensitive enough to reveal differences within this species.