Abstract: The purpose of the present study was to investigate the preventive effect of different Molecular Weight (MW) fragments from dietary fiber Corn Bran Hemicellulose (CBH) on hepatitis induced by D-galactosamine in rats in relation to their degradation and fermentation in gastrointestinal tract. Rats fed the diets supplemented with the fragments with or without neomycin sulfate in experiment 1 and the diets supplemented with Short Chain Fatty Acids (SCFAs) in experiment 2 were administered with D-galactosamine intraperitoneally. The serum transaminase activities, cecal and fecal total and reducing sugar content, cecal SCFAs were analysed. Among the fragments tested, the oligosaccharide fragments with MW ranging from 285.7 to 930.1 significantly suppressed the enhancement of serum transaminase levels caused by D-galactosamine. The ingested oligosaccharide fragments produced striking amount of SCFAs in cecum particularly propionate formation. However, SCFAs tested in experiment 2 could not suppress the elevation of serum transaminase levels by D-galactosamine. The present study demonstrated that the oligosaccharide fragments were effective in preventing D-galactosamine hepatitis and the effect might not be mediated by the action of SCFAs.